Journal: Pharmacological research

Article Title: Modulation of cellular bioenergetics by CO-releasing molecules and NO-donors inhibits the interaction of cancer cells with human lung microvascular endothelial cells.

doi: 10.1016/j.phrs.2018.09.005

Figure Lengend Snippet: Fig. 4. Effect of CORM-401 and PAPA NONOate on MDA-MB-231-luc2-tdTomato cell transendothelial migration and adhesion to lung microvascular endothelial cells.

Article Snippet: Human lung microvascular endothelial cell line (hLMVEC) was purchased from the European Cell Culture Collection (Cell Applications, San Diego, CA, USA), human breast adenocarcinoma MDA-MB-231-luc2-tdTomato cell line stably expressing the firefly luciferase gene and tdTomato fluorescent protein was kindly provided by Prof. Joanna Wietrzyk (Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences). hLMVEC cells were maintained in Microvascular Endothelial Cell Growth Medium (Cell Applications), MDA-MB-231-luc2-tdTomato cells were cultured in RPMI1640 GlutaMAX Medium (Gibco) with 10% FBS (Gibco), AAS (Sigma-Aldrich) containing 20 units of penicillin, 20 mg streptomycin and 0.05 mg amphotericin B.

Techniques: Migration